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. 2014 Apr 8;57(8):3235–3246. doi: 10.1021/jm401605c

Figure 6.

Figure 6

Compound 8 inhibits MARCKS phosphorylation, cell proliferation, and invasion following treatment with 8. (A) HeLa cells were starved for 16 h in medium without fetal calf serum. Subsequently, they were treated with 50 μM solution of compound 8 for 30 min and 100 nM TPA for the last 10 min. An amount of 150 μg of protein from lysed cells was loaded onto SDS 8–16% gels. Tubulin was used as loading control. Below a representative blot, a bar graph corresponding to quantitative scans of three independent experiments (±SD) is shown. (B) Cell proliferation following treatment of HeLa and PC-3 cells with 8. The mean values (±SD) of three independent experiments, in which three samples were taken within each experiment, are shown. (C) Invasion of cells through a membrane. Cell invasion was determined as described in Experimental Section. In HeLa cells with a doxycycline-inducible PKCε the expression of PKCε was induced with doxycycline (control + Dox). The mean of three experiments (±SD) is shown: (∗) p = 0.03. The data are normalized to the control (100%). Therefore, the standard deviation for the control is 0.