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Polymerase chain reaction (PCR) analyses with each primer set and different amount of genomic DNA of C. sinensis (5 pg to 50 ng) were performed. The primer sets used were the same as previously described. Lane 1, 50 ng; lane 2, 5 ng; lane 3, 500 pg; lane 4, 50 pg; lane 5, 5 pg.
Sensitivity assay.
