. 2012 May;106(2):82–93. doi: 10.1179/2047773212Y.0000000016

Table 2. Genes identified in the MAGs of An. gambiae.

Functional class	Anopheles	Drosophila	Aedes	Expression data (%)
Acps
	AGAP001510	nd	AAEL017145	100
	AGAP006362	CG13699	AAEL014767	100
	AGAP006581	Acp62F*	AAEL000356	13
	AGAP006583	Acp63F*	nd	100
	AGAP006585	Acp63F*	nd	100
	AGAP006586	Acp62F*	AAEL000356	17
	AGAP006587	Acp62F*	nd	100
	AGAP006589	nd	nd	100
	AGAP008116	nd	AAEL010264	100
	AGAP008968	CG31704	nd	nd
	AGAP009352 (AGAP012681)	Acp70A*	nd	100
	AGAP009353 (AGAP012680)	Msopa*	nd	100
	AGAP009354	Mst57Da*	nd	100
	AGAP009355	Dro-PA	nd	100
	AGAP009356	Mst57Da*	nd	100
	AGAP009357	Dro-PA	nd	100
	AGAP009358 (AGAP012682/AGAP012830)	Nplp4	nd	100
	AGAP009359	Mst57Da*	nd	100
	AGAP009360 (AGAP012807)	CG13230	nd	98
	AGAP009361	Acp95EF*	nd	nd
	AGAP009362	CG6409	nd	100
	AGAP009367	Acp26Ab*	nd	100
	AGAP009368	CG14770	nd	100
	AGAP009369	Acp53Ea*	nd	100
	AGAP009370 (AGAP012706)	Acp53Ea*	nd	100
	AGAP009371	CG14302	nd	100
	AGAP009372	CG32726	nd	nd
	AGAP009373	vsg	Supp02310*	100
	AGAP013714	nd	nd	100
	AGAP013731	CG13230	nd	98
	AGAP013734	CG15065	nd	100
	AGAP013776	Nplp4	nd	100
Redox
	AGAP001039	spo	AAEL009762	100
	AGAP003067	Cyp304a1	AAEL014413	100
	AGAP005784	PAM	AAEL007732♮	100
	AGAP007420	PHM	AAEL001394	0
	AGAP007491	CG4670	AAEL012054	35
	AGAP008019	Cyp12b2	AAEL002031♮	99
	AGAP008203	Cyp6a2	AAEL009120	100
	AGAP009363	Cyp9f2	AAEL001312♮	0
	AGAP009584	Trx-2	AAEL010777	24
	AGAP012855	Cyp6a2	AAEL009120	100
	CYP302A1	dib	AAEL015655	85
	CYP306A1	phm	AAEL004888	88
	CYP314A1	shd	AAEL011850	98
	CYP315A1	sad	AAEL010946	100
Proteases
	AGAP005791	CG12951	nd	100
	AGAP005792	CG12951	nd	100
	AGAP008276	Try29F	nd	100
	AGAP008277	Try29F	nd	100
	AGAP008997	CG8172	AAEL000238	100
	AGAP012315	CG34290	AAEL012447♮	100
	AGAP013150	CG9806*	AAEL009108	100
	ENSP017764	nd	nd	100
	ZCP1	nd	nd	100
	ZCP3	nd	nd	100
	ZCP4	nd	nd	100
	ZCP6	nd	nd	nd
	ZCP7	nd	nd	100
	ZCP9	nd	nd	100
Nucleic acid binding
	AGAP000355	Mkrn1	AAEL007476	100
	AGAP000754	CG15439	AAEL003032	100
	AGAP000916	pAbp	nd	100
	AGAP000918	pAbp	nd	100
	AGAP000920	pAbp	nd	100
	AGAP003844	cwo	AAEL010513	100
	AGAP009339	CG6654	AAEL005029	100
	AGAP009699	sens-2	AAEL001243	100
	AGAP010358	gsb	nd	100
	AGAP010359	prd	nd	100
Hydrolases
	AGAP001649	CG31414	AAEL014321♮	100
	AGAP005255	Rab3	AAEL006267	100
	AGAP006425	CG9701	nd	100
	AGAP010720	pom1	AAEL013237	100
	COEBE1D	EST-6	nd	nd
	COEBE4D	EST-7	nd	nd
Protein folding
	AGAP000831	CG7872	AAEL013114	100
	AGAP001424	CG5520	AAEL012827	nd
	AGAP001502	CG11267	AAEL001052	10
	AGAP007088	CG2852	AAEL013279	3
	AGAP008822	CG9847	AAEL004313	0
	AGAP012407	PDI*	AAEL000641*	4
Transferases
	AGAP000843	CG4774	AAEL012719♮	100
	AGAP009099	CG7356	nd	100
	AGAP009190	CG4688	AAEL007955	100
	AGAP009191	CG16936	AAEL007954	100
	AGAP009365	CG5973	AAEL001297	100
	AGAP009377	CG5973	AAEL001297	93
Protein binding
	AGAP000927	sqh	AAEL008921	100
	AGAP004761	mwh	AAEL008269	100
	AGAP005684	Syx17	AAEL000282♮	100
	AGAP007041	fibrinogen	AAEL001713*	100
	AGAP012986	spi	AAEL011205	100
Transmembrane proteins
	AGAP000107	pyx	AAEL004179	98
	AGAP002824	Takr86C	AAEL017414	100
	AGAP010637	Toll6	nd	100
	AGAP010861	CG1698	AAEL003626	100
Immune peptides
	AGAP007049	CG10433	AAEL009861	2
	AGAP009429	Anp*	nd	98
	TEP15	CG10363	AAEL014755	nd
Serpins
	AGAP005246	CG9334	AAEL007765	17
	SRPN9	CG10956	AAEL008364	nd
Nucleic acid metabolism
	AGAP000139	tam	AAEL015671	100
	AGAP009842	CG8194	AAEL001159	36
Protein metabolism
	AGAP000926	l(1)G0196	AAEL008950♮	100
	AGAP009673	QPCT	AAEL010727	100
Lipases
	AGAP003083	CG17097	AAEL012343♮	100
	AGAP003749	CG6296	AAEL012790	100
CRISPs
	AGAP006418	CG17575*	AAEL009239*♮	100
Kinases
	AGAP002181	CG5644	AAEL010062	100
Others
	AGAP004428	CG3359	AAEL014917	3
	AGAP005239	CG8323	AAEL006262	93
	AGAP005504	scramb1	AAEL010661	100
	AGAP007339	TpnC47D	AAEL000744	96
	AGAP009001	Hdc	AAEL014632	100
	AGAP009189	Eps-15	AAEL007950	100
	AGAP009364	CG5793	AAEL001308	0
	CALRETICULIN	Crc	AAEL001005♮	nd
Unknown
	AGAP003736	CG30053	nd	100
	AGAP005859	nd	nd	100
	AGAP008439	CG31705	AAEL005017	100

Notes:</emph> Acps, accessory gland proteins; CRISPs, cysteine-rich secretory proteins.

The table contains all 121 genes identified to date in the accessory glands of An. gambiae (Anopheles) males, identified by a number of strategies (as described in the text).⁶⁸^–⁷¹ Genes are organized based on their functional class. When known, the putative D. melanogaster (Drosophila) and Ae. aegypti (Aedes) orthologues are indicated. In the Anopheles column, identifiers in bold represent genes whose products have been detected in the male accessory glands (MAGs) by mass spectrometry⁶⁸ and/or reverse transcription PCR (RT-PCR) data.⁶⁸^–⁷⁰ The ‘*’ symbol indicates D. melanogaster and Ae. aegypti genes that are expressed in the MAGs of these species. The ‘♮’ symbol in the Aedes column indicates the presence of multiple putative orthologues (only the most similar orthologue is reported). The expression data column refers to the degree of MAG specificity of the An. gambiae genes, as obtained from MozAtlas website (http://www.tissue-atlas.org/); the degree of specificity is reported as the percentage of expression observed in the MAGs relative to all male and female tissues where expression was present in a minimum of 3/4 calls.⁷¹ Numbers are rounded to the nearest integer. Numbers in bold represent expression data obtained by transcriptional and/or immuno blotting analyses.⁶⁸^–⁷⁰ Please note that An. gambiae genes in the following groups share a common probeset in mozatlas: (1) AGAP009354, AGAP009356, AGAP009359; (2) AGAP009355, AGAP009357; (3) AGAP009358, AGAP0013776; (4) AGAP009360, AGAP0013731; (5) AGAP009365, AGAP009377; and (6) AGAP009369, AGAP009370.