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. 2012 Mar;106(1):40–45. doi: 10.1179/2047773212Y.0000000001

Table 1. Sequences of primers used for detection of rickettsial DNA by nested PCR.

Gene Target detected Primers Primer sequence (5′–3′) Product size (bp)
gltA6.7 All rickettsiae (typhus group and SFGR) RpCS.877p GGGGACCTGCTCACGGCGG 381
RpCS.1258n ATTGCAAAAAGTACAGTGAACC
RpCS.896p GGCTAATGAAGCAGTGATAA 338
RpCS.1233n GCGACGGTATACCCATAGC
17 kDa8,9 All SFGR R17122 CAGAGTGCTATGAACAAACAAGG
R17500 CTTGCCATTGCCCATCAGGTTG
Tz 15 TTC TCA ATT CGG TAA GGG C 246
Tz 16 ATA TTG ACC AGT GCT ATT TC
ompA10 All SFGR except Rickettsia helvetica Rr190k.71p TGGCCAATATTTCTCCAAAA 650
Rr190k.720n TGCATTTGTATTACCTATTGT
Rr190 k.71p TGGCGAATATTTCTCCAAAA 532
Rr190k.602n AGTGCAGCATTCGCTCCCCCT
ompB11 All SFGR Rc.rompB.4362p GTCAGCGTTACTTCTTCGATGC 475
Rc.rompB.4836n CCGTACTCCATCTTAGCATCAG
Rc.rompB.4496p CCAATGGCAGGACTTAGCTACT 267
Rc.rompB.4762n AGGCTGGCTGATACACGGAGTAA