Table 1. Sequences of primers used for detection of rickettsial DNA by nested PCR.
Gene | Target detected | Primers | Primer sequence (5′–3′) | Product size (bp) |
gltA6.7 | All rickettsiae (typhus group and SFGR) | RpCS.877p | GGGGACCTGCTCACGGCGG | 381 |
RpCS.1258n | ATTGCAAAAAGTACAGTGAACC | |||
RpCS.896p | GGCTAATGAAGCAGTGATAA | 338 | ||
RpCS.1233n | GCGACGGTATACCCATAGC | |||
17 kDa8,9 | All SFGR | R17122 | CAGAGTGCTATGAACAAACAAGG | … |
R17500 | CTTGCCATTGCCCATCAGGTTG | |||
Tz 15 | TTC TCA ATT CGG TAA GGG C | 246 | ||
Tz 16 | ATA TTG ACC AGT GCT ATT TC | |||
ompA10 | All SFGR except Rickettsia helvetica | Rr190k.71p | TGGCCAATATTTCTCCAAAA | 650 |
Rr190k.720n | TGCATTTGTATTACCTATTGT | |||
Rr190 k.71p | TGGCGAATATTTCTCCAAAA | 532 | ||
Rr190k.602n | AGTGCAGCATTCGCTCCCCCT | |||
ompB11 | All SFGR | Rc.rompB.4362p | GTCAGCGTTACTTCTTCGATGC | 475 |
Rc.rompB.4836n | CCGTACTCCATCTTAGCATCAG | |||
Rc.rompB.4496p | CCAATGGCAGGACTTAGCTACT | 267 | ||
Rc.rompB.4762n | AGGCTGGCTGATACACGGAGTAA |