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. 2014 Apr 1;124(5):1987–1999. doi: 10.1172/JCI70324

Figure 6. Mct8/Oatp1c1 DKO mice display reduced myelination.

Figure 6

(A) Frontal vibratome brain sections of male animals (n = 3 per genotype and time point) were stained with FluoroMyelin (FM), a dye that incorporates into myelin sheaths. Additionally, consecutive sections were stained with an antibody against MBP. Quantification of the fluorescence signal densities revealed reduced staining in Mct8/Oatp1c1 DKO mice at all time points (P12, P33, and P120). Scale bar: 600 μm. (B) Ultrathin sections of the medial part of the cc were analyzed by electron microscopy at P21 (n = 3). Compared with WT animals, the number of myelinated axons was visibly decreased in Mct8/Oatp1c1 DKO mice as well as in athyroid Pax8 KO mice. However, the ultrastructure of the myelin sheaths appeared rather similar in all genotypes (higher-magnification insets). Scale bars: 5 μm (top); 500 nm (bottom); 50 nm (insets). (C) P21 and P180 coronal paraffin brain sections subjected to Gallyas silver staining were used to quantify cc thickness at the cingulum bundle (n = 3). Only Mct8/Oatp1c1 DKO mice showed decreased cc thickness at either time point. *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT, or as otherwise indicated (brackets).