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. 2014 Apr 1;124(5):1987–1999. doi: 10.1172/JCI70324

Figure 8. Abnormal gait and severe locomotor deficiencies in Mct8/Oatp1c1 DKO mice.

Figure 8

Mct8/Oatp1c1 DKO animals exhibited abnormal gait, as demonstrated by significantly reduced stride length (A), and a strongly elevated hind paw angle (B) based on footprint analysis. (C) Locomotor deficiencies were monitored by a rotarod test using 4-month-old male mice (n = 6–8 per genotype). In contrast to WT, Mct8 KO, and Oatp1c1 KO animals, Mct8/Oatp1c1 DKO mice performed poorly on the rod and did not show a learning curve. #P < 0.001 vs. WT. (D) In order to assess balance and coordination, mice were monitored while running on a beam 1 cm wide and 100 cm long. Recording the hind limb slips revealed a significantly higher number of errors only in Mct8/Oatp1c1 DKO mice. (E) Mice were further subjected to a hanging wire test in order to determine neuromuscular abnormalities. Only Mct8/Oatp1c1 DKO mice were not able to cling on a metal wire for 60 seconds. (F) As these findings point to reduced muscle performance, forepaw muscle strength was quantified using a grip strength meter, revealing an approximately 35% reduction in Mct8/Oatp1c1 DKO animals. *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT, or as otherwise indicated (brackets).