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. 2014 Apr 8;124(5):2099–2112. doi: 10.1172/JCI71643

Figure 5. Hypoxia inhibits β-adrenergic signaling.

Figure 5

(A) Relative copy number of Adrb1 and Adrb3 transcript expression in BAT under these experimental conditions (n = 4–5). (B) Relative copy number of Adrb1 and Adrb3 transcript expression in BAT of the KO and WT mice (n = 3–8). (C and D) Western blot analysis of phosphorylated PKA (pPKA Thr197) and total PKA in BAT from mice fed NC or HFHS diet or BAT from aP2-Cre+/– Vegfaflox/flox (KO) and control Vegfaflox/flox mice (WT). Right graphs indicate quantification relative to PKA (for pPKA) and GAPDH-loading control (for PKA) (n = 3). (E) Graphical illustration of the downregulation of adrenergic signaling under conditions of obesity and the proposed positive feedback loop caused by hypoxic conditions. Data were analyzed by 2-tailed Student’s t test (C and D) or ANOVA (A and B). *P < 0.05; **P < 0.01. All values represent the mean ± SEM.