Figure 2. DMF treatment reduces neutrophil infiltration into the spinal cords in wild-type mice but not in Hca2^–/– mice with EAE.

The number of (A) CD45⁺CD4⁺ T helper cells, (B) CD45⁺CD8⁺ T cytotoxic cells, (C) CD45⁺CD11b⁺ macrophages, (D) CD45⁺Ly-6G⁺ neutrophils, and (E) CD45⁺CD11c⁺ dendritic cells in the spinal cord was quantified by flow cytometry on dpi 17. DMF or vehicle were given orally (30 mg/kg body weight, twice per day) starting from dpi 3. Results are expressed relative to the vehicle group of the same genotype. Data are mean ± SEM from 14 to 15 mice per group. *P < 0.05 (Student’s t test with Bonferroni correction).