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. 2014 Apr 8;124(5):2037–2049. doi: 10.1172/JCI72522

Figure 3. Effects of knockdown of EPHA2 on a panel of NSCLC cell lines.

Figure 3

(A) 14 NSCLC cell lines were transduced with lentiviruses containing either shEPHA2 or a pLKO.1 vector control. The resulting cell populations were selected in 1 to 2 μg/ml puromycin for 5 days. Cell viability was analyzed by the MTT assay at 72 hours after puromycin selection. Experiments were repeated twice with 6 replicates per experiment. Data were pooled and are presented as viability of shEPHA2 knockdown cells relative to that of the vector control cells ± SEM. (B) Immunoblotting for EPHA2 expression confirmed knockdown in 8 NSCLC cell lines. β-Tubulin expression was used as a loading control. (C) Cells were treated as in A, and cell viability was assessed over 5 days. Experiments were repeated twice, and data were pooled and are presented as shRNA knockdown relative to the vector control cells ± SEM.