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. 2014 Mar 25;124(5):2246–2259. doi: 10.1172/JCI73639

Figure 3. Recognition and lysis of autologous tumor by CD8+ TILs sorted based on PD-1, LAG-3, and TIM-3 expression.

Figure 3

Bulk CD3+CD8+ TILs were sorted to high purity from FrTu3289, FrTu3612, FrTu3713, FrTu3550, and FrTu2448 based on positive or negative expression of PD-1, LAG-3 and/or TIM-3, and expanded in vitro for 15 days. (A–E) Response of fresh tumor–derived TILs to their respective autologous tumor cell lines, TC3289 (A), TC3612 (B), TC3713 (C), TC3550 (D) and TC2448 (E). Reactivity was assessed by measuring IFN-γ release (duplicates, mean ± SD) and frequency of 4-1BB upregulation. (F–H) Cytolytic activity of fresh tumor–derived TILs in response to their respective autologous tumor cell lines, TC3289 (F), TC3713 (G), and TC2448 (H). Percentage of specific lysis at different effector/target ratios is shown as mean ± SD.