Figure 3.
Results of Leishmania diagnostic and four species-specific PCR tests17 for the 25 samples listed in Table 1. PCRs are listed on the left, with their specificity between brackets. The size of the internal controls (IC) and the Leishmania specific (L) fragments are indicated by arrows on the right. The GeneRuler 100-bp molecular weight marker (Fermentas, St Leon-Rot, Germany) is used as size reference (M), of which the region between 100 and 500 bp is depicted. The negative no-template controls (N) are shown in the first lane of the gels, where only the IC fragment is amplified.