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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Curr Opin Plant Biol. 2014 Mar 15;0:87–95. doi: 10.1016/j.pbi.2014.02.008

Figure 2.

Figure 2

MIRNA hairpins produce more than one product.

A. Schematic of a typical MIRNA locus with aligned reads from small RNA-seq, and explanation of terms and calculations.

B. Distribution of precisionann values from Arabidopsis MIRNA loci with respect to miRBase 20. Based on genome alignment of a small RNA-seq dataset comprised of NCBI GEO GSM738731 and GSM738727.

C. As in B, except for precisionmax values.

D. Frequency of concordance between miRBase 20 annotations of the mature miRNA, and the observed most abundant RNA for the small RNA-seq data.

E. As in B, except using small RNAs from an AGO1-IP experiment (NCBI GEO GSM989351), and restricting the analysis to MIRNA loci annotated with a mature miRNA 21 nt in length with a 5′ U.

F. As in E, except for precisionmax values..

G. Frequency of concordance as in D, except for AGO1-IP data and restricting the analysis to MIRNA loci annotated with a mature miRNA 21 nt in length with a 5′ U.