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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Gastroenterology. 2014 Feb 4;146(5):1339–1350.e1. doi: 10.1053/j.gastro.2014.01.061

Figure 4.

Figure 4

Macrophage depletion abrogates fibrosis resolution. MAFIA mice were subjected to sham, 2 weeks BDL, or 2 weeks BDL plus CJ for 5 days. In situ GFP (200×) showed CSFR1-positive cell infiltration in scar tissue during fibrosis resolution in MAFIA mice in absence of AP-20187 (A). After CJ + BDL, MAFIA mice were treated with vehicle or AP20187 daily for additional 5 days. Peripheral blood was collected and subject to fluorescence-activated cell sorting analysis. CD 11b and GFP double-positive cells were significantly decreased after AP20187 treatment compared with vehicle (B). GFP-positive cells were also significantly decreased in livers of MAFIA mice treated with AP20187 compared with vehicle (C). Sirius Red staining (D) showed attenuated fibrosis resolution (5 days after CJ) after macrophage depletion. (Ve, vehicle; AP, AP 20187; n = 7; P < .05).

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