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. 2014 Mar 31;24(6):564–576. doi: 10.1093/glycob/cwu025

Fig. 7.

Fig. 7.

Characterization of lipid II in B. cenocepacia. (A) Ascending paper chromatography of cell pellets of the strain YFM2 (Prha::murJBc, ΔlysA) under permissive (rhamnose) and non-permissive (glucose) conditions showing accumulation of cell wall precursors under non-permissive conditions. This experiment is representative of three independent experiments showing accumulation of lipid II at average Rf value of 0.47. (B) Accumulation of extracted lipid II from aqueous phase of the strain YFM2 under non-permissive conditions. Data correspond to the average of CPM ± SD of three independent experiments. n = 3. P-value = 0.0247. (C) MALDI-TOF MS of lipid II of E. coli W3110. D. MALDI-TOF MS of lipid II of B. cenocepacia K56-2. (C) and (D) are representative spectra of three independent preparations of lipid II.