Suppression of Src-family PTK signaling disrupts the distribution of Par-3, but not Rho B. The distribution of known markers of oocyte polarity Par-3 and Rho B was determined by confocal immunofluorescence analysis of DMSO-treated CF1 oocytes (control, panels A,B), Fyn-null oocytes (panels C,D), or SKI-606-treated CF1 oocytes (panels E,F). Fixed oocytes were labeled with anti-Par-3 (A,C,E) or anti Rho B (B,D,F) followed by alexa 488-conjugated secondary antibody. Magnification is indicated by the bar which represents 10 mm.