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. Author manuscript; available in PMC: 2014 Apr 28.
Published in final edited form as: Biopolymers. 2010;94(5):659–664. doi: 10.1002/bip.21416

FIGURE 5.

FIGURE 5

Time course of cleavage of a synthetic phalloidin precursor (substrate: MSDINATRLPAWLATCPCAGDD). Purified POP from C. apala was incubated with the substrate for the indicated times. Products were separated by HPLC and quantitated by their absorbance at 280 nm. The intermediate was identified as MSDI-NATRLPAWLATCP and the product as AWLATCP by mass spectrometry. No evidence for a carboxy-terminal intermediate (AWLATCPCAGDD) was found. Dithiothreitol was included in the enzyme buffer to prevent oxidation of the two Cys residues (Originally published in Luo et al. J Biol Chem 2009, 284:18070–18077. © the American Society for Biochemistry and Molecular Biology).