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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Virus Res. 2014 Jan 30;183:30–40. doi: 10.1016/j.virusres.2014.01.018

Figure 7. The MRV M3 mRNA 3′ UTR is not necessary for efficient translation initiation or start site selection in MRV T1L core transfected cells.

Figure 7

A) M3 si-01 targets nts 194–212 of the T1L strain of MRV. The corresponding nucleotide and amino acid sequences are depicted. Mismatched bases in the wobble base rescue construct (T1L-WB) are in boldface and are underlined in the gene sequence. BsrT7 cells were transfected or mock transfected with M3 si-01. AT 6 h p.t., CsCl purified MRV T1L core particles (4×108 particles) were transfected with or without WT or mutant T1L-WB rescue plasmids. At 24 hrs post transfection cells were harvested and clarified lysates were analyzed by Immunoblotting to detect. B) μNS and μNSC proteins or D) MRV proteins. C) MS si-01 treated L929 cells transfected with MRV T1L core particles with or without WT or mut T1L-WB rescue RNAs were harvested at 0, 24, and 48 h p.t. by repeated freeze-thaw cycles (−80°C and 37°C), and relative viral titers were determined by conducting plaque assays on L929 mouse fibroblasts and expressed as log10 (relative viral titer).