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. 2014 Mar 5;592(Pt 8):1839–1856. doi: 10.1113/jphysiol.2013.268706

Figure 2. Location of NTS microinjection site.

Figure 2

A, representative images show coronal sections of the brainstem of a rat showing the sites of successful microinjection with colloidal gold in the caudal region of the NTS. Aqueous Eosin Y is used as a cytoplasmic counterstain. Magnification to 6.3×. B, sample time course of ventilatory responses to 50 μl NMDA microinjections in two different rats. Animals were not used for further measurements or analysis if they showed no response to AMPA, NMDA or glutamate microinjection (negative localization). AP, area postrema; CC, central canal; GR, gracile nucleus; NTS, nucleus tractus solitarius.