Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb;143(2):203–214. doi: 10.1085/jgp.201311094

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Bellono et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — IP₃R and TRPA1 activity mediate UVR-induced Ca²⁺ responses and regulate signaling kinetics. (A) In HEMs the amplitudes of the retinal-dependent Ca²⁺ responses induced by 240 mJ/cm² UVR in the presences of vehicle (1% DMSO) were reduced by the IP₃R antagonist XeC (25 µM) and the TRPA1 antagonist HC-030031 (HC; 100 µM), and further reduced in the presence of both XeC and HC. n = 10–15 cells per condition. (B) Mean peak UVR-induced Ca²⁺ responses in the presence of vehicle were reduced by ∼71% in the presence of XeC, by ∼45% in the presence of HC, and by ∼90% by the coapplication of XeC and HC, respectively. n = 6 experiments per condition. P < 0.0001 for XeC versus control, P < 0.007 for HC versus control, P < 0.0001 for XeC + HC versus control, ±SEM (error bars). (C) Analysis of the first 30 s of the UVR-induced Ca²⁺ response showed that treatment with XeC or HC reduced the initial slope compared to vehicle control. n = 10–15 cells from one experiment per condition. The broken lines represent the slopes of the initial increase in calcium (the straight line that fits the initial phase of the calcium response curve). The tangents of the angles formed by these lines with the x axis are represented in D (ΔF/s). (D) The mean initial slope of the Ca²⁺ response measured during the first 30 s after the beginning of UVR stimulation was reduced by ∼76% when cells were treated with XeC and by ∼35% when cells were treated with HC, compared with vehicle-treated cells. n = 6 experiments per condition. P < 0.0001 for XeC versus control, P < 0.03 for HC versus control, ±SEM (error bars). (E) The decay of the UVR-induced Ca²⁺ response (τ_off), calculated by fitting the responses with a single exponential, was significantly longer in HEMs treated with XeC and reduced in HEMs treated with HC, compared with vehicle-treated cells. n = 10–15 cells per condition. The broken lines represent the exponential curve that fits the decreasing part of the calcium response shown in A. The exponential coefficient of these curves represents τ_off, shown in F. (F) The mean τ_off of UVR-induced Ca²⁺ response decay in HEMs treated with vehicle was increased by ∼79% in the presence of XeC and reduced by ∼38% in the presence of HC. n = 6 experiments per condition. P < 0.03 for XeC versus control, P < 0.03 for HC versus control, ±SEM (error bars).