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. Author manuscript; available in PMC: 2014 Apr 28.
Published in final edited form as: Toxicol Sci. 2008 Apr 14;104(1):135–143. doi: 10.1093/toxsci/kfn078

Figure 2. Dose-response analysis.

Figure 2

Real –time PCR analysis of Serca2a (2A and B) and Ryr2 (2C and D) transcripts was performed in H9c2 cells exposed to different doses of TCE (10 and 100 ppb, 1 and 10 ppm) for either 12 (A and C) or 48 hours (B and D). Fold expression at each dose was calculated against control (without TCE) as a ratio between Serca2a and B-actin. Graphs in A and B represent the average of three independent experiments. Each experiment point was tested in triplicate. [*] identify significant differences as determined by Student’s t-test (p < 0.05). Graphs in C and D represent the average of two independent experiments, each one was repeated at least two times.