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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Immunol. 2014 Mar 31;192(9):4453–4464. doi: 10.4049/jimmunol.1400146

Fig. 3. Lung priming modulates the alveolar inflammatory milieu.

Fig. 3

(A) Apoptosis (Annexin V+/7-AAD+) of BAL neutrophils from primed and non-primed WT mice on days 1, 3, or 5 after i.t. LPS was assessed by flow cytometry. BAL neutrophils were gated by characteristic granulocyte forward and side scatter, sub-gated for Gr-1+ to identify neutrophils, and then for AnnexinV/7-AAD, percentages for which are quantified in (A). BAL TNF-α (B), IL-10 (C), and active TGF-β1 (D) cytokine secretion were assessed at designated time points after i.t. LPS in primed and non-primed WT mice. Values expressed as mean ± SEM; *paired t-test against other group at same time point, p<0.05. (n=4-6 animals per group per time point)