| Dilution | Volume of MNase (µl) |
MNase source |
Volume of Tris pH 8.0 (µl) |
|---|---|---|---|
| A | 1.0 | stock | 14.0 |
| B | 9.6 | Dilution A | 5.4 |
| C | 7.5 | Dilution B | 7.5 |
| D | 7.5 | Dilution C | 7.5 |
| E | 7.5 | Dilution D | 7.5 |
| F | 7.5 | Dilution E | 7.5 |
| G | 7.5 | Dilution F | 7.5 |
| H | 0.0 | - | 15.0 |
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