Table 1. Validation of cDNA Microarray Results.
Selected genes showing at least a 2-fold differential expression between SCC25-PD and SCC25-WD cells by cDNA microarray analysis (9) were validated by triplicate real time reverse-transcriptase PCR. Relative expression levels were normalized to the housekeeping gene PGK1. All genes in the panel showed at least a 2-fold upregulation (p<0.05).
Gene | Fold Change (SCC25-PD vs. SCC25-WD) |
---|---|
IL8 | 87.4 +/− 27.0 |
IL1A | 64.1 +/− 8.0 |
PTGS2 | 25.2 +/− 3.0 |
TNFAIP3 | 15.5 +/− 1.0 |
ICAM1 | 7.8 +/− 3.1 |
SOD2 | 6.7 +/− 1.9 |