Figure 3.

(a) Annotated schematic illustration of vacuum molding method for construct assembly. b) Fluorescence imaging of patterned multiphase constructs using mixed and pure microbead preparations. Schematic shows intended pattern and mosaic reconstruction of fluorescent images shows the construct created. c) Images of layered constructs created using vacuum molding. d) Fluorescent imaging of graded microbead constructs containing red- and green-labeled fibroblast cells and day 1 and day 7 in culture. Schematic shows approximation of intended gradient across construct. Insets show higher magnification view of embedded cells.