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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Feb 6;34(4):837–845. doi: 10.1161/ATVBAHA.113.302335

Figure 2. Tamoxifen inducible Tie2-Cre-mediated inactivation of Ppap2b results in loss of endothelial LPP3 and impairs angiogenesis.

Figure 2

(A) Matrigel plugs supplemented with bFGF were implanted in Ppap2bfl/fl (designated as fl/fl) and ERT2-Ppap2bΔ (designated as ERT2-Δ) mice treated with tamoxifen. Seven days later, animals were injected with FITC-dextran to visualize blood vessels. Sections of the explanted plugs were stained with antibodies to LPP3 (red) and PECAM (blue) to identify endothelial cells. (Mag 40X, Bar denotes 100μm). Matrigel sections from ERT2-Δ mice lacked endothelial LPP3 staining. (B) Representative whole mount images of FITC-dextran in explanted Matrigel plug from Ppap2bfl/fl (fl/fl) and ERT2-Ppap2bΔ (ERT2-Δ) mice taken at a lower magnification (10X) demonstrate that a more extensive vascular network forms in the fl/fl control mice. (C) Quantification of vessel size in the Matrigel implants from Ppap2bfl/fl (fl/fl) and ERT2-Ppap2bΔ (ERT2-Δ) mice demonstrates that smaller vessels form in the absence of LPP3. * P < 0.0001 by Fisher’s exact test.