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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Parasite Immunol. 2013 Dec;35(12):409–420. doi: 10.1111/pim.12049

Figure 8.

Figure 8

L. major infection inhibits ETS-mediated transcription. RAW 264.7 cells stably transfected with an ETS luciferase reporter were infected with L. major for 16 hr followed by LPS stimulation for 8 hours. Samples were analyzed in triplicate. *p≤0.05 (Wilcoxon Matched Pair test; n=7) compared to unstimulated control. One representative of three independent experiments is presented. B) WT and CD11b KO BMMP were infected 10:1 for 16 hrs, after which cells were lysed and assayed for ETS-2 mRNA expression by real-time PCR. Data was normalized to GAPDH and fold change was calculated by the ΔΔCt method, calibrated to uninfected samples. *p≤ 0.05 (Student’s paired t-test; n=3). Samples were analyzed in triplicate.