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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mech Dev. 2014 Feb 28;132:1–12. doi: 10.1016/j.mod.2014.02.004

Fig. 6. FoxD3 is required for neural crest specification independent of Tspan18.

Fig. 6

Whole mount images of embryos unilaterally co-electroporated with FoxD3MO and either ContMO (A,C) or TS18MO (B,D) at late gastrula, incubated to 5–6 somites (s), and processed by in situ hybridization for Sox10 (A,B) or Snail2 (C,D). (A-D) Co-electroporation of FoxD3MO with either ContMO or TS18MO disrupts Sox10 (A,B) and Snail2 (C,D) expression on the targeted side of the neural tube (asterisks; green in A’-D’). (E,F) Bar graphs representing the frequency of electroporated embryos that exhibit a severe, moderate, mild, or no reduction in Sox10 (E) or Snail2 (F) expression, indicating that Tspan18 knockdown has no impact on the severity of FoxD3-dependent specification defects.