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. 2011 Apr 5;32(4):528–536. doi: 10.1038/aps.2010.235

Table 3. The enzymatic activities of other antioxidant enzymes of different cell groups 48 h after RNAi.

Cell lines Enzymatic activity Blank Prx1 siNeg Prx1 siRNA Prx5 shNeg Prx5 shRNA Combined Neg Combined
Eca-109 CAT 33.70±0.70 32.60±2.61 34.23±2.01 33.01±2.20 32.90±2.60 31.77±1.90 33.87±1.31
  GPx 1.88±0.29 1.74±0.57 1.63±0.34 1.59±0.29 1.84±0.38 1.37±0.30 1.78±0.34
  Mn-SOD 4.48±0.79 4.24±0.52 3.98±0.60 4.48±0.21 4.38±0.18 4.12±0.44 3.66±0.27
  Cu/Zn-SOD 2.94±0.47 3.23±0.51 2.05±0.49 2.87±0.59 2.70±0.55 2.13±0.52 2.64±0.22
TE-1 CAT 33.63±2.94 33.33±3.73 31.43±2.00 32.87±2.06 33.90±1.30 34.03±2.34 33.23±2.39
  GPx 1.92±0.66 1.86±0.54 1.57±0.25 1.75±0.20 1.94±0.62 1.67±0.25 1.97±0.76
  Mn-SOD 4.78±0.23 4.00±0.64 4.39±0.20 4.03±0.09 4.45±0.45 3.99±0.42 3.82±0.35
  Cu/Zn-SOD 3.06±0.50 3.08±0.44 3.01±0.71 3.19±0.31 2.84±0.62 2.92±0.33 2.38±0.16

Abbreviations are: CAT, catalase; SOD, Mn- and Cu/Zn- superoxide dismutase; GPx, glutathione peroxidase.

*

SOD: U/mg protein; CAT, μmol/L H2O2 per min per mg protein; GPx: μmol/L NADPH per min per mg protein.