Figure 2.

Apoptosis induced by triptolide. (A) After treatment with triptolide at 80 nmol/L for 12 h, cells were collected and detected by the annexin-V/propidium iodide (PI)-staining method. The results were expressed as mean±SD (n=3). (B) After treatment with triptolide at indicated concentrations for 12 h, Molt-4 cells were stained with Hoechst 33258 and then observed under a fluorescence microscope. Arrows, apoptotic nuclei. (C) Leukemia cell lines were incubated with 80 nmol/L of triptolide for 12 h, and then cell extractions were subjected to Western blotting analysis using anti-caspase-3 and PARP antibodies. The results are the representatives of three separate experiments.