Figure 6.
Suppressive effects of p38 MAPK inhibitor, SB203580 (A) and PI3K inhibitor, LY294002 (B) on LPS-induced IL-8 mRNA level in HUVECs. Cells were pretreated with SB203580 (25 μmol/L) or LY294002 (50 μmol/L) for 1 h and then exposed to LPS (100 ng/mL) for 4 h. After treatment, the mRNA expression of IL-8 was analyzed by RT-PCR as described in Materials and methods. Data are representative of three experiments (mans±SD). cP<0.01 compared to the vehicle-treated group; eP<0.05, fP<0.01 compared to the LPS-treated group.