FIGURE 5.

H2ABbd-mediated apoptosis was blocked by caspase inhibitor and NF-κB inhibitor. A, HeLa cells were treated with or without Dox and benzyloxycarbonyl-VAD-fluoromethyl ketone (Z-VAD) (10 μm), a pancaspase inhibitor, for 48 h. Total cell extracts were prepared for immunoblotting. B, HeLa cells were treated with or without Dox, benzyloxycarbonyl-VAD-fluoromethyl ketone (10 μm), or ASA (20 mm), an inhibitor of NF-κB, for 48 h. As a control, HeLa cells were irradiated with UV (100 J/m²) and cultured with or without ASA (20 mm) for 48 h. Typical differential interference-contrast images and sub-G₁ DNA contents (percentages) analyzed by FACS are shown. C, HeLa cells were treated with or without Dox and ASA (20 mm) for 48 h. Total cell extracts were prepared for immunoblotting. D, HeLa cells were irradiated with UV (100 J/m²) and cultured with or without ASA for 48 h, and total cell extracts were prepared and analyzed by immunoblotting.