FIGURE 5.

Effective mechanism of Sj7170 on the cell proliferation of U87 cells. A, Western blot analysis of cyclin D1, cyclin D2, cyclin D3, CDK2, CDK4, CDK6, cyclin E1, cyclin E2, Rb2/p130, E2F1, E2F5, p21, and p27 in control and Sj7170-treated U87 cells. c, control. B, inhibition of cyclin D1 expression by cyclin D1 shRNAs in U87 cells. Real time PCR and Western blot were used to examine the mRNA and protein expression levels of cyclin D1. C, effects of Sj7170 on cell proliferation in U87 cells by knockdown of cyclin D1. D, subcellular localization of Sj7170 in U87 after 1 and 24 h of incubation. Immunofluorescence results of Sj7170 in U87 cells are shown. Blue indicates DAPI nuclear staining. Green fluorescence was plotted in the cytoplasm as location of Sj7170 in U87 cells.