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The X-band continuous wave EPR spectra of the disemiquinoid forms of nNOS. A, nNOSred; B, nNOSred + NADP+; C, nNOSred + CaM; D, nNOSred + CaM + NADP+; E, R1400E nNOSred + CaM + NADP+; F, full-length nNOS; and G, full-length nNOS + CaM. Experimental parameters: microwave power, 10 μW; modulation amplitude, 1 G; temperature, 80 K.
