FIGURE 8.

The hydrostatic pressure dependence of single-turnover NO formation by full-length nNOS. NO formation was detected using oxyhemoglobin as described under “Materials and Methods.” A, progress curves, corrected for background absorbance, obtained at 401 nm for nNOS in the presence of CaM at 1 bar (black, solid line), 1500 bar (black, dashed line), and nNOS in the absence of CaM at 1 bar (red, solid line) and 1500 bar (red, dashed line). B, pressure dependence of NO formation for full-length nNOS in the presence of CaM. The open gray circle represents the observed rate after de-pressurizing the sample. The error bars represent the S.D. calculated from three individual transients.