FIGURE 7.

ClpC₆₈₀₃ forms an active proteolytic complex with ClpP1-ClpR and degrades the adaptor proteins NblA1₆₈₀₃ and NblA2₆₈₀₃. A, in vitro degradation of α-casein. α-Casein was incubated in the presence of pyruvate kinase (20 ng/μl), 4 mm phosphoenolpyruvate, 2 mm ATP, and purified ClpC₆₈₀₃ and ClpP1/R (2 μm each) at 37 °C. Samples were taken at the indicated time points, separated by SDS-PAGE, and visualized by Coomassie Blue R-250 staining. B, in vitro degradation of purified NblA1₆₈₀₃ and NblA2₆₈₀₃. NblA1₆₈₀₃/NblA2₆₈₀₃ (5 μm each) were incubated in the presence of pyruvate kinase (20 ng/μl), 4 mm phosphoenolpyruvate, 2 mm ATP, purified ClpC₆₈₀₃ and ClpP1-ClpR (2 μm each), and purified PBS from Synechocystis 6803 (29) at 37 °C. Samples were taken at the indicated time points, separated by SDS-PAGE, and visualized by Coomassie Blue R-250 staining.