TABLE 2.
Chromatin immunoprecipitation primer sequences
kB represents the number of kilobases away from the transcription start site the KLF4 binding site, CACCC, is located. The corresponding primer sequence flanks the CACCC site in that region on the target gene promoter.
| Gene | Forward sequence | Reverse sequence |
|---|---|---|
| VEGFA (−3.2 kB) | gccttggcccaatcactac | gtggagaaaagtgcgaaagg |
| VEGFA (−3.1 kB) | gaacatttgggaaatctcttgc | tgtggctctgaaggcagtt |
| VEGFA (−0.6 kB) | gcagacggcagtcactagg | cactctcgaagacgctgct |
| VEGFR1 (−3.7 kB) | agccaatggcgatcttataaac | ctcccgtctgctcctgact |
| VEGFR1 (−0.2 kB) | ttctctgagctgatggaggac | ggcttggactgtgataagaagg |
| VEGFR2 (−0.1 kB) | cctccgcgctctagagttt | cacacattgaccgctctcc |
| VEGFR2 (+0.1 kB) | cgcagaaagtccgtctgg | gacagttgagcgcacagg |
| NOTCH1 (−5.0 kB) | ctctccctgttgcccagat | aggatcagtggcgtcgtg |
| NOTCH1 (−3.8 kB) | gatggccgtccagatgac | cccccaataaggcacaaaa |
| NOTCH1 (−3.2 kB) | ctacctgggcagcctcaa | ttcttcctccgtgccttg |
| NOTCH1 (−2.9 kB) | atccagcagcagcaaagc | ctgagctcacgccaaggt |
| DLL4 (−4.8 kB) | ttctttctctcctgctgacca | ttcgccacatgagaagcat |
| DLL4 (−3.8 kB) | ccccacctagctttcctctc | ccgcagacaaatgctcataa |
| DLL4 (−3.4 kB) | tcaatgtgaatggatcgaatg | acagaaggcgtggagatttg |
| DLL4 (−2.6 kB) | gaaggccgcaagtgtttct | gaaggccgcaagtgtttct |
| DLL4 (−2.4 kB) | tcagcaaatgtgggcaaag | attcccgggctaggacact |
| DLL4 (−0.8 kB) | tgggctgacagaaacacttg | cgagctttgcccacattt |
| DLL4 (−0.5 kB) | gggccagaacctcattacc | gtggtcccggaggtagga |
| HES1 (−3.6 kB) | ctggcgaaatcattgacaac | cacggttctcagacacttcatt |
| HES1 (−2.8 kB) | ctaagtgcggtcaggcatc | gcctccaagtttgctcctc |
| HES1 (−2.4 kB) | tgcgtgtgtgagtgagtgtg | attaactggcagagtggcaggtaa |
| HES1 (−2.2 kB) | cgcacacacactcactcaca | cgtgccaggatgttttattg |
| HES1 (−0.5 kB) | tgagagagggcataaagagca | tttttctttccgctttggtc |