FIGURE 1.

FSP27 depletion increased both basal and stimulated lipolysis in human adipocytes. Nonspecific scrambled (Scr) siRNA was used as a control in all experiments. A, relative mRNA levels in siRNA-transfected human adipocytes. B, immunoblot analysis and quantification of protein expression levels of FSP27 and β-tubulin (loading control) of siRNA-transfected human adipocytes. C, biochemical quantification of basal and stimulated lipolysis on the basis of measurement of glycerol release after 2 h. Data are mean ± S.E. *, p < 0.05; **, p < 0.001, n = 3 (unpaired Student's t test).