FIGURE 4.

FSP27-mediated TG accumulation was ATGL-dependent. Biochemical quantification of total triglycerides in human adipocytes treated with scrambled siRNA (Scr, control) (A) or siRNA against ATGL in the presence or absence of adenoviral expression of FSP27-HA or EGFP (as a negative control) and adipocytes differentiated from WT or ATGL-KO MEFs (B). FSP27-HA or EGFP (control) were expressed using adenovirus (adV). Total triglyceride concentration was normalized to total protein. Data are mean ± S.E. (paired Student's t test). *p < 0.001, and **p < 0.05, n = 3.