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. 2014 Mar 13;289(17):12029–12039. doi: 10.1074/jbc.M113.539890

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — FSP27 (120–220) protected against FFA-induced insulin resistance in human adipocytes. Shown is insulin-stimulated AKT phosphorylation in human adipocytes. The core FSP27domain associated with TG accumulation, aa 120–220, was expressed using lentivirus, with EGFP as a control. The cells were treated overnight with 100 μm PA/BSA. The blots show AKT phosphorylation in basal and insulin-stimulated conditions. FSP27 (120–220) protected human adipocytes from inhibition of AKT phosphorylation by exogenous PA.