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Schematic representation of the reaction mechanisms of asparagine synthetase A and aspartyl-tRNA synthetase enzymes. Step 1 involves activation of the selective carboxyl groups by the respective enzymes to form aminoacyl-AMP. Step 2 involves amidation of the aminoacyl-AMP by asparagine synthetase A and transfer of the activated amino acid to the cognate tRNA by aspartyl-tRNA synthetase.
