FIGURE 2.

ManR and ASGR mRNA levels increase in pseudopregnant mice. Virgin female mice were mated with vasectomized male mice. The presence of a vaginal plug was utilized to assign day 1 of pseudopregnancy. On day 4 of pseudopregnancy, oil was injected in the uterine horn of a subset of the bred mice. On day 9 of the pseudopregnancy, the mice were sacrificed, and their livers were collected for analysis of mRNA levels by qPCR. A, ManR; B, Asgr-1; C, Asgr-2. The results are shown for: virgin mice (Virgin), pseudopregnant mice that were not further manipulated (Bred), pseudopregnant mice treated with oil that did not develop decidua (No Decidua), and pseudopregnant mice treated with oil that developed decidua (Decidua). In each case, the level of message differed significantly (p < 0.05) from that found in virgin mice using the unpaired t test. Error bars, S.E. with n = 4.