FIGURE 4.

Progesterone increases expression of the ManR and ASGR in the liver of female mice. Progesterone pellets (2.5 mg/pellet) were implanted subcutaneously in female mice. After 72 h, the mice were sacrificed, and livers were collected. Following homogenization, membranes were prepared by sedimentation on a sucrose cushion. Ten μg of total protein was loaded onto each lane for analysis by SDS-PAGE. Following electrophoresis, the proteins were electrophoretically transferred to PVDF membranes, and the amount of ManR (A) and ASGR (B) was determined by Western blot analysis using antibodies specific for the ManR and ASGR, respectively. The intensity of each band was determined using ImageJ software. The bar graphs show the mean for three treated or three control mice with error bars = S.E. Lanes 1–3 and white bars indicate control mice. Lanes 4–6 and solid black bars indicate progesterone-treated mice.