FIGURE 6.

The ManR accounts for clearance of glycoproteins bearing N-linked glycans terminating with either β1,4-linked GalAc-4-SO₄ or α-linked Man. GLuc-PSG23 was expressed in CHO/T3 cells, CHO/T3 cells co-transfected with GalNAc-4-ST1, or CHO/Lec1 cells to generate N-linked glycans terminating with GalNAcβ1,4GlcNAcβ1,2Man (GLuc-PSG23:GalNAc) (A), SO₄-4-GalNAcβ1,4GlcNAcβ1,2Man (GLuc-PSG23:SO₄-4-GalNAc) (B), and Man (GLuc-PSG23:Man) (C), respectively. WT, ASGR-deficient (ASGR^−/−), and ManR-deficient (ManR^−/−) male mice were injected with the GLuc-PSG23 constructs indicated, and samples were collected immediately, then at 1-min intervals for 5 min, 2.5-min intervals from 7.5 to 15 min, and 5-min intervals from 15 to 25 min. The amount of GLuc activity present in the equivalent of 320 nl of plasma was determined for each time point after dilution of plasma aliquots. Individual time points were analyzed in triplicate, and error bars = S.E. but are not visible because they are smaller than the symbols used. LU, light units.