FIGURE 7.

Increased expression of the ASGR and ManR during pregnancy increases the rate of GLuc-PSG23 clearance from the blood. Gluc-PSG23 was expressed in CHO cells (A), CHO/T3 cells co-transfected with GalNAc-4-ST1 (B), CHO cells and digested with neuraminidase prior injection (C), CHO cells co-transfected with α1,3-fucosyltransferase-9 (D), CHO/T3 cells (E), and CHOT3 cells co-transfected with α1,3-fucosyltransferase-9 to generate N-glycans with the termini shown (F). The GLuc-PSG23 products were injected into WT female mice on day 15 of pregnancy. Blood was collected immediately and at the times shown. The amount of GLuc activity present in the equivalent of 320 nl of plasma was determined for each data point following dilution of aliquots. The light units (LU) of activity in samples from pregnant animals were corrected for the 50% great blood volume seen during pregnancy. Individual time points were analyzed in triplicate, and error bars = S.E. but are not visible because they are smaller than the symbols used.