TABLE 1.
Primers used for PCR
| Gene/Construct primer | Primer sequences (5′-3′) | Accession no. |
|---|---|---|
| Hprt1 | NM 013556 | |
| Probe | CTTGCTGGTGAAAAGGACCTCTCGAA | |
| Forward | AACAAAGTCTGGCCTGTATCC | |
| Reverse | CCCCAAAATGGTTAAGGTTGC | |
| MR1 (Mrc1) | NM 008625 | |
| Probe | ACGAAATCCCTGCTACTGAACCTCC | |
| Forward | ATGGATGTTGATGGCTACTGG | |
| Reverse | TTCTGACTCTGGACACTTGC | |
| Asgr-1 | NM 009714 | |
| Probe | TGACTGAAGCTCTGAAAGGACCTGC | |
| Forward | GAGTCGAAGCTGGAAAAACAG | |
| Reverse | CCTTCATACTCCACCCAGTTG | |
| Asgr-2 | NM 007493 | |
| Probe | ATTGCCCCGAAATGCAGCCAC | |
| Forward | ACGTGAAGCAGTTAGTGTCTG | |
| Reverse | CCTTCATACTCCACCCAGTTG | |
| GLucPSG23 | ||
| Gau3′-PSG23-5′-AS-B | ACGTGTTGTGGTGACTGGATGGTGACACTGTCACCACCGGCCCCCTTGATC | |
| Gaussia-3′-S-r | GATCAAGGGGGCCGGTGGTGArC | |
| PSG23-3′-MYC-5′-S-B | TCCCAGTCAGCCTGGCCGTGATGAATGAGTCTAGAGGGCCCGAACAAAAACTCATCTC | |
| LMYC-AS-r | GTCACCAGTGGAACCTGGAACrC | |
| Gaussia-3′-AS-r | GTCACCACCGGCCCCCTTGATrC | |
| LMYC-S-r | GAGGGCCCGAACAAAAACTCATCTrC | |
| mFut9 | ||
| mFUT9-BamHI-S | CGAGCTCGGATCCATGACATCAACATCCAAAGG | |
| mfut9-EcoRI-as | TGCAGAATTCCATTAATTCCAAAACCATTTCTCTAAATTACC | |