FIGURE 1.

Physical interaction between OGT and TLEs. A, coimmunoprecipitation analysis. COS-7 cells were transfected with HA-tagged OGT or His-tagged TLE1 or -2 expression vectors and immunoprecipitated with α-HA antibody, followed by immunoblotting with α-HA and α-His antibodies. B–E, mapping interaction domains in OGT and TLEs. TLE proteins and their deletion mutants were synthesized in reticulocyte lysate and were incubated with bacterially produced full-length OGT and deletion mutants. F, schematic representation of primary structures of TLE and OGT and the identified interactions. CcN, a region containing phosphorylation sites for Cdc2 and casein kinase 2; SP, serine/proline-rich domain; WD, WD40-repeat domains in tandem.