(a) Molecular design and construction of the recombinant S4E8G
protein polymers consisting of tandemly repeated elastin-like pentapeptide
sequence GVGVP and silk-like hexapeptide sequence GAGAGS. The trigger
temperatures for thermal response of the Au-S4E8G NPs were genetically
engineered to between 50 and 60 °C (b). Identity (b) and purity
of the recombinant S4E8Gs, containing four different numbers of silk-elastin-like
repeats: 3-, 8-, 13-, and 18-mer, were characterized by mass spectrometry
and (c) SDS-PAGE.