Antitumor effects. (a) Tumor growth curves,
drug IV administration:
once a day on day −1, illumination 24 h postdrug administration
[hv#: 100 mW/cm2 for 30 min (180 J/cm2) or 200 mW/cm2 for 30 min (360 J/cm2)], 5
mice per group except the control group (4 mice). Error bars represent
SE. In inset: the order of tumor size was G1 > G3 > G4 during
day
1 to day 4 [** p < 0.01 (G1 vs G3, from day 1
to day 4) and ## p < 0.01 (G3 vs G4, from day
1 to day 4)]. (b) Kaplan–Meier plot of response to treatment.
(c) H&E staining of tumors 24 h post-illumination from (i) control
mice or mice treated with (ii) Pc-(NCL-CA4)2, or (iii)
Pc-(L-CA4)2. Prodrug administration and illumination conditions
were same as for those in (a). VT, viable tumor; DT, damaged tumor;
and DS, damaged skin.