. 2014 Mar 26;57(8):3532–3545. doi: 10.1021/jm500268j

Table 1. Relative Binding Affinities (RBAs) of Compounds 2 and 4–6 for Estrogen Receptor α and β^a.

graphic file with name jm-2014-00268j_0005.jpg

Determined by a competitive radiometric binding assay with [³H]estradiol; preparations of purified, full-length human ERα and ERβ (Invitrogen) were used; see the Experimental Section. Values are reported as the mean ± the range or SD of two or more independent experiments; the K_d for estradiol for ERα is 0.2 nM and for ERβ, 0.5 nM. K_i values for the reported compounds can be readily calculated using the formula K_i = (K_d[estradiol]/RBA)100.

Table 1. Relative Binding Affinities (RBAs) of Compounds 2 and 4–6 for Estrogen Receptor α and βa.

Table 1. Relative Binding Affinities (RBAs) of Compounds 2 and 4–6 for Estrogen Receptor α and β^a.