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. 2013 Sep 16;11(1):49–57. doi: 10.1038/cmi.2013.40

Figure 1.

Figure 1

DHX33 senses both poly I:C and an RNA virus in D2SC mDCs. (a) IB showing the knockdown efficiency of shRNAs targeting the indicated proteins in D2SC cells. Scrambled shRNA served as a control (left most lane). Actin blots are shown as loading controls (lower panel). ELISA of IFN-α and IFN-β production by D2SC cells treated with the indicated shRNAs after stimulation with (b) 2.5 µg/ml long poly I:C, (c) 5 µg/ml short poly I:C or (d) reovirus. Cells were stimulated with nucleic acids, delivered by Lipofectamine 2000, for 16 h. Virus was added to the cells at a MOI of 10 for 2 h. Cells were collected, washed with PBS and cultured for an additional 16 h. (e) ELISA of TNF-α and IL-6 production by D2SC cells treated with the indicated shRNAs after 16 h of stimulation with 1 µg/ml LPS. N-STM, unstimulated D2SC cells treated with scrambled shRNA. Individual circles represent the value from each independent experiment. Bars represent the average values from at least three independent experiments. IB, immunoblot; IFN, interferon; LPS, lipopolysaccharide; MOI, multiplicity of infection; PBS, phosphate-buffered saline; shRNA, small heteroduplex RNA.